Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 474-2

474-2

FIRST CASE REPORT OF Corynebacterium pseudoberculosis BIOVAR EQUI IN SEMIARID REGION OF BRAZIL

Autores:

Gutiele do Nascimento do É (UNIVASF - Universidade Federal do Vale do São Francisco) ; Isabelle Caroline Pires (UNIVASF - Universidade Federal do Vale do São Francisco) ; Danillo Sales Rosa (UNIVASF - Universidade Federal do Vale do São Francisco) ; Nayara Andreo (UNIVASF - Universidade Federal do Vale do São Francisco) ; Artur Cezar de Carvalho Fernandes (UFPB - Universidade Federal da Paraíba) ; Ruy Brayner de Oliveira Filho (UFPB - Universidade Federal da Paraíba) ; Flavia Figueira Aburjaile (UFMG - Universidade Federal de Minas Gerais) ; Mateus Matiuzzi da Costa (UNIVASF - Universidade Federal do Vale do São Francisco)

Resumo:

This is the first report of an equi biovar in Brazil, identifying the semiarid Northeast region. Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis in small ruminants that causes significant global economic damage. In addition, it causes pyogranulomatous reactions, ulcerative lymphangitis, mastitis, necrotic and ulcerative dermatitis in mammals a horse. Aspiration puncture of the subcutaneous abscesses was performed, and the samples were sent to the Laboratory of Preventive Veterinary Medicine (LMVP / UFPB) and the Laboratory of Infectious Diseases (LDIC-UFRPE) for bacteriological analysis tests. The microbiological diagnosis was carried out with culture, isolation, and identification of the microorganism from the material obtained from the abscesses by aspiration puncture and seeded on 5% sheep blood agar. Biochemical tests were performed from the isolated colonies to confirm the species, with positive catalase, negative oxidase, positive urea, and nitrate results. After Gram staining, the microscopy revealed gram-positive, confirmation of the Corynebacterium genus, these samples were sent for molecular diagnosis through the MALDI TOF methodology to confirm the bacterial species and genomic sequencing using the HiSeq 2500 paired-end platform (Illumina, San Diego, CA, USA). The taxonomic analyses classified the genome by average nucleotide identity (ANI), genome distance matrix (MinHash tool) and the OrthoFinder. The nitrate reduction biochemical test classified the biovariety in equi, which was confirmed as the sequencing and assembly of the complete genome 2,369,947 bp with 52.1%GC content. The new strain was named Cp_Eq_BR01 and deposited with Bioproject PRJNA857423, Biosample SAMN29625301 and Genbank SUB11780237. Based on nucleotide comparative analyzes with other genomes of C. pseudotuberculosis, the results highlight the significant similarity between them (> 98%) and the clonal characteristics of these genomes are evident, mainly between isolates from the same region and host. Although the C. pseudotuberculosis equi had never been reported before, biovar ovis is widespread in small ruminants. Therefore, the disease requires better surveillance in horses with a more accurate and faster diagnosis for immediate and effective treatment. The genomic analysis reveals high similarity between all C. pseudotuberculosis biovars equi and its connection with the host and geographic location.

Palavras-chave:

diagnosis, lymphangitis, pathology, zoonoses

Agência de fomento:

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The Pernambuco Science and Technology Support Foundation (FACEPE).